Validation of a β-ME ELISA for Detection of Anti Leishmania donovani Antibodies in Eastern Sudan
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Abstract:
Background: A β-mercaptoethnol (β-ME)-treated promastigote antigen of L. donovani was successfully employed in direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis (VL). Objective: The β-ME-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-ME ELISA) and evaluated for VL diagnosis against outcome of reference freeze-dried DAT (FD-DAT) and rK39 strip test (RKT) commercial kits. Methods: Two-hundred and ninety-two sera from patients with high VL suspicion of whom 105 had confirmed L. donovani infection were tested. Results: Relatively higher sensitivities of 93.3% (95% CI: 88.4- 98.2) and 92.4% (95% CI: 87.3-97.5) were determined for β-ME ELISA and FD-DAT as compared to 83.8% (95% CI: 76.7-90.8) for RKT. Of 73 VL sera that scored maximum absorbance values (>0.81) in β-ME ELISA, 66 (90.4%) tested at the highest agglutination titres (>1:51200) in FD-DAT as did 56 (76.7%) also at comparable reaction intensities (3 + colour intensity) in RKT. Compared with FD-DAT (94.7%, 95% CI: 91.5-97.9) or RKT (93.0%, 95% CI: 89.3-96.6), lower specificity was estimated for β-ME ELISA (90.4%, 95% CI: 86.1-94.6). Based both on positive and negative microscopy for L. donovani in organ aspirates of all VL suspects enrolled (292), significantly higher correlation (p
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validation of a β-me elisa for detection of anti leishmania donovani antibodies in eastern sudan
background: a β-mercaptoethnol (β-me)-treated promastigote antigen of l. donovani was successfully employed in direct agglutination test (dat) for the diagnosis of visceral leishmaniasis (vl). objective: the β-me-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-me elisa) and evaluated for vl diagnosis against outcome of reference freeze-dried dat (fd-...
full textValidation of a β-ME ELISA for detection of anti Leishmania donovani antibodies in Eastern Sudan.
BACKGROUND A β-mercaptoethnol (β-ME)-treated promastigote antigen of L. donovani was successfully employed in direct agglutination test (DAT) for the diagnosis of visceral leishmaniasis (VL). OBJECTIVE The β-ME-treated antigen was further incorporated into an enzyme-linked immunosorbent assay set-up (β-ME ELISA) and evaluated for VL diagnosis against outcome of reference freeze-dried DAT (FD-...
full textQualitative and Semi-Quantitative Comparison of an Rk39 Strip Test and Direct Agglutination Test for Detection of Anti-Leishmania Donovani Antibodies in the Sudan
Background: Until now, the comparison of the rK39 strip test (RKT) and direct ag-glutination test (DAT) for detection of visceral leishmaniasis (VL) is exclusively based on either positive or negative qualification of the reaction outcome. Objective: In this study, we compared the diagnostic performance of RKT and DAT for VL both qualitatively and semi-quantitatively. Methods: For comparison ba...
full textDetection of antibodies to Leishmania donovani in animals in a kala-azar endemic region in eastern Sudan: a preliminary report.
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Journal title
volume 8 issue 3
pages 150- 158
publication date 2011-09-01
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